/EXTE Environmental Impacts of Exhaust Gas Cleaning Systems for Reduction of SOx on Ships — Analysis of status quo 
zeport compiled within the framework of the project ImpEx 
Table A-5: Results of research studies on ecotoxicological effects of EGCS discharge water 
Research study | Tested water 
Koski et al. (2017) 
Samples taken on board from an OL 
system and HFO with 2.5% sulphur 
content. 
MLIT (2018) 
Produced in laboratory from exhaust 
from a four-cylinder 257 kW engine, 
HFO with 2.2% sulphur content and 
hybrid system operated in OL mode 
(+47 m?/MWh). Dissolved oxygen and 
pH were not adjusted during sample 
preparation 
Magnusson et al. 
(2018) 
Samples taken on board from OL and 
CL systems 
Ytreberg et al. 
{2019) 
Produced in laboratory from exhaust 
from a four-cvlinder 100 kW engine, 
Analysis and results 
ı Algal growth inhibition (Rhodomons sp., no standard reported) 
o At 100% exposure — 100% inhibition 
Oo At 10% exposure — increase growth compared to control 
» Zooplanktonic copepod (Acartia tonsa, no standard reported) 
5 Lethal effects analysed — adult (LCso between 20-30%) and egg mortality (LCso >50%) as a function of 
EGCS discharge water exposure 
Sublethal effects analysed —- feeding and reproduction as a function of EGCS discharge water and 
dietary exposure 
ı Algal growth inhibition — acute toxicity (Skeletonema costatum, ISO 10253) using growth rate after 72 
hours as the endpoint 
LCso of 49% (48“50%) 
5 NOEC of 32% and LOEC of 100% 
» Crustacean — acute toxicity (Hyale barbicornis, US EPA OPPTS 850.1020) using mortality rate after 96 
hours as the endpoint 
5 LCso of 20% (12.5725%), the acute effects were expressed immediately 
o NOEC of 12.5% and LOEC of 25% 
* Fish — acute toxicity (Oryzias javanicus, OCDE TG203) using mortality rate after 96 hours as the endpoint 
5 LCso of 35% (25%750%), the acute effects were expressed immediatelv 
o NOEC of 25% and LOEC of 50% 
ı Luminescent bacteria — acute toxicity (Vibrio fischeri, ISO 11348-3) 
5 ICso of 15.5% with CL discharge water 
» Zooplanktonic copepod medium term -—- chronic toxicity (Calanus helgolandicus, non-standard species), 7- 
14 days exposure time 
5 Toxic effects (mortality) at 0.04-0.1% with CL discharge water 
5 Toxic effects (mortality) at 1.0% with OL discharge water 
Blue mussel —- chronic toxicity (Mytilus edulis, non-standard species), 15-35 days exposure time 
5 Toxic effects (byssus strength) at 1.25% with CL discharge water 
o No observed effects with OL discharge water 
ı« Mesocosm experiment (microplankteon communitv, non-standard species) 
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