22 
BSH Report No. 56 
Laboratory Performance RSD (n=4) 
(Based on Peak Heights of Hopanes and Steranes) 
29bbR 
28bbS 
28bbR 
27bbS 
27bbR 
—BSH_BioM 
Fig. 12; RSD of the biomarker groups hopanes and steranes, based on the peak heights (raw 
counts). 
The RSD values In the radar chart are based on raw counts, expecting that 
normalisation compensates for variance If the main Impact Is of a systematic 
nature. Consider discussion point DP-3 on page 31. 
Nevertheless, looking at the conclusion matrix (Tab. 4), It Is surprising that 
matching ratios from the group of the steranes are succeeding those of the 
hopanes by far. 
On the other hand, positive matches are suggested for samples of set 2 from 
both, RWS and BSH analysis, which contradicts the results from FID and 
MS-85m/z conclusions. This observation continues Into the duplicate analyses, 
where hopanes show a non-matching tendency and steranes suggest a 100% 
match. 
These results lead to the conclusion that the blomarkers are of very limited use 
for this purpose, without further preparation or concentration of the samples 
only leads to Inconclusive and erratic results.