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Eukaryotic Biodiversity and Spatial 
Patterns in the Clarion-Clipperton 
Zone and Other Abyssal Regions: 
Insights From Sediment DNA and 
RNA Metabarcoding 
Franck Lejzerowicz*?*, Andrew John Gooday®*, Ines Barrenechea Angeles®®, 
Tristan Cordier*”, Raphael Morard®, Apotheloz-Perret-Gentil Laure®, Lidia Lins’® 
Lenaick Menot’’', Angelika Brandt’%", Lisa Ann Levin'*, Pedro Martinez Arbizu?s 
Craig Randall Smith'® and Jan Pawlowski*? 17 
V1V2 Eukaryota 
PC2 (43.12 %) 
Nagrertertomyesien 
Chrysophyceae« 
Hydrozoa 
/ Parmales 
_ (Bolidophycopae) 
pa. 
2 
- 
Chaetoceros 
°O223— 
CCZ area 
BGR 
BGR North 
IFREMER 
OMS 
UK-1B 
80-0 
= BOL2-7IKG 
fr 
BRL2-SKC 
 BIOL2-56KG 
° 
BDL2-5XG 
Min 
Km 
Region 
CZ 
Mid Atlantic 
North Atlantic 
Northwest Pacific 
South Atlantic 
Southern Ocear- 
-*0223— 
Cercozoa 
Filofeta sp! 
tEndomyxaf 
Endomyxa- 
Ascetosporea ) Cercozoa 
| Eukaryota 
896 _ samn!- 
PC1 (47.43 %) 
South Atlantic 
Molecule type 
"{ona | ]rnA 
Southern Ocean 
) 
r 
FIGURE 1 | Location of the sequenced sediment samples. Samples in the Pacific Ocean include two regions: the CCZ (five different areas, see inset) and the 
Northwest Pacific (Kuril-Kamchatka area). Samples in the Atlantic Ocean include three regions (North, Mid, and South Atlantic), as well as three areas (and stations) 
in the Southern Ocean region.