Figure Captions 
Figure 1. Scatterplots showing the output from each analytic tool for the > 50 pm size class 
compared to total microscopy estimates. Panel (a) shows full data while panel (b) shows the 
subset of data corresponding to microscopy estimates below 40 individuals per ml. All values 
are standardized to represent the concentration of organisms (or biomass indicator) in the 
prepared subsamples. The solid line indicates the line of best fit found using Deming regression 
(formula indicated above graph) and dashed lines (where applicable) indicate 1:1 line. Number 
of data points (n) is indicated above each plot. Note different x-axis limits in panel (a) 
corresponding to available data. Red dots are used for FDA methods; green dots indicate CFA 
methods, and blue dots indicate ATP methods. 
Figure 2. Scatterplots showing the output from each analytic tool for the 10-50 pm size class 
compared to total microscopy estimates. Panel (a) shows full data while panel (b) shows the 
subset of data corresponding to microscopy estimates below 40 individuals per ml. All values 
are standardized to represent the concentration of organisms (or biomass indicator) in the 
prepared subsamples. The solid line indicates the line of best fit found using Deming regression 
(formula indicated above graph) and dashed lines (where applicable) indicate 1:1 line. Number 
of data points (n) is indicated above each plot. Note different x-axis limits in panel (a) 
corresponding to available data. Red dots are used for FDA methods; green dots indicate CFA 
methods, blue dots indicate ATP methods; purple dots indicate flow cytometry. Black dots 
represent points below detection limit of tool; points are plotted at minimum detection level 
(Hach BW680 only). 
Figure 3. Scatterplots showing pairwise comparisons of raw Fv values for each CFA device and 
microscopy counts (ind/mL) for the 10-50 pm size class. The Pearson correlation coefficient (r) 
is indicated for each pair.